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On cell number) was used for cytospin preparation. Slides were air-dried for 30 min, fixed O/N in 10 formalin, then stored in 70 ethanol at 4 , until IHC staining. IHC staining was performed using Ventana Benchmark ULTRA automated slide stainers, and antibodies against pan keratin (Neomarkers, Fremont,CA, USA, MS-343-P), EpCAM (DAKO, Heverlee, Belgium, M0804) and p53 (DAKO, M7001).Pathway ana
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