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Acer (ITS) region was performed using 10 pmoles of forward and reverse primers (ITS1 forward primer TCCGTAGGT GAACCTGCGG and ITS4-reverse primer TCCTCCGC TTA TTGATATGC [35], 25 L of Qiagen SYBR master mix (Qiagen, Australia) and 5 L of template in a total 50 L reaction mix. Cycling was performed using the following conditions: 95 for 15 min, 45 cycles of 95 for 30 sec, 50 for 30 sec and 72 for

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